UBA7 Knockout cell line (THP-1)

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Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Cell Monoclonal Culture Medium

Monoclone Genotype Validation Kit

UBA7 Knockout cell line (THP-1) Order

Catalog#

YKO-H1004

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

UBA7 Knockout cell line (THP-1)

Morphology

Monocyte,suspension

Passage ratio

1：2-1：4

Species

Human

Gene Name

UBA7

Gene ID

7318

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Culture method

90%RPMI-1640+10%FBS

Target region

Knockout region: Exon 3 (based on transcript UBA7-201)
Validation: PCR+Sanger Sequencing

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:THP-1			STR Info (Cell bank) Cell Line:THP-1
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	11	12		11	12
D13S317	13	13		13	13
D7S820	10	10		10	10
D16S539	11	12		11	12
VWA	16	16		16	16
TH01	8	9.3		8	9.3
AMEL	X	Y		X	Y
TPOX	8	11		8	11
CSF1PO	11	13		11	13

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the UBA7 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the THP-1 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, UBA7 gene knockout UBA7 Knockout cell line (THP-1) will be expanded and cryopreserved.

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